HIV P24- envelope hybrid antigen (HIV Type 2)

HIV p24-envelope hybrid antigen (HIV Type 2)

E.coli-based expression of  isolated HIV envelope sequences  poses problems : The polypeptide of interest typically exhibits high hydrophobicity and insolubility in aqueous media and a low yield in bacterial expression systems, all of which results  in a poor degree of purity. These difficulties can be circumvented by designing a composite  HIV recombinant polypeptide by which the envelope  gene segment is inserted into the HIV p24 antigen gene sequence and expressed as a single hybrid polypeptide.


HIV p24-envelope hybrid antigen (HIV Type 2)



Viral variants tend to exhibit significant sequence diversity in their envelope segment, making immunoassays solely dependent on epitopes from this region unreliable. Combining the p24 core antigen with a selected high-impact segment from the envelope would therefore make sense, and our solution is indeed patented technology:

This protein is a 258 amino acid fusion protein, supplied in frozen format and consisting of the Type 1 HIV p24 core polypeptide with a conserved, highly immunoreactive envelope polypeptide sequence derived from HIV-2 envelope inserted at the gene level. The protein  has been designed with a similar strategy as that employed to develop the p24-env (Type 1) antigen described above, but now carries a specific, conserved structure from the HIV Type 2 transmembrane protein. The expressed hybrid molecule has been subjected to extensive purification produces a single band on SDS and is soluble in physiological buffer. The HIV Type 2 p24 env recombinant polypeptide contains no non-HIV related sequences. It has proven highly effective in detecting HIV Type 2 antibodies in sera from individuals infected with this virus. This protein requires judicious application of the purification protocol and is typically obtained in somewhat lower yield than its Type 1 counterpart.

(Purchase of the protein carries an implicit license for its use as a diagnostic source ingredient)

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